What is FCS format?

FCS stands for “Flow Cytometry Standard.” The goal of the standard is to provide a uniform file format that allows files created by one type of acquisition hardware and software to be read by any other (Spidlen). The FCS file provides a detailed description of: instrument used to obtain the data.

What is FSC a measure of?

The measurement of forward scatter allows for the discrimination of cells by size. FSC intensity is proportional to the diameter of the cell, and is primarily due to light diffraction around the cell. Forward scatter is detected by a photodiode, which converts the light into an electrical signal.

What is SSC A and FSC A?

forward scatter area (FSC-A) density plot can be used to exclude doublets as shown in Figure 3 below. A side scatter height (SSC-H) vs side scatter area (SSC-A) plot can also be used.

Can flow cytometry be wrong?

Trummer and colleagues demonstrated that using isotype negative controls can induce errors: they concluded that using flow cytometry to discriminate between positive and negative populations of MPs labeled with several antibodies could be an obstacle for their characterization [79].

How do I open a FlowJo file?

To load your files into FlowJo, drag-and-drop a folder of files onto the Workspace window, or click the Add Sample button to get a file navigation window, browse to your folder of files and click Choose. Note that FlowJo will automatically create groups based on the folder structure and names.