What is Taq polymerase used for in PCR?

What is Taq polymerase used for in PCR?

Due to its key role in synthesizing and amplifying new strands of DNA, Taq DNA Polymerase is essential to Polymerase Chain Reaction (PCR). Like other DNA polymerases, Taq Polymerase can only produce DNA if it has a primer, a short sequence of 20 nucleotides that provide a starting point for DNA synthesis.

Who makes Taq polymerase?

Hoffmann-La Roche
Hoffmann-La Roche eventually bought the PCR and Taq patents from Cetus for $330 million, from which it may have received up to $2 billion in royalties. In 1989, Science Magazine named Taq polymerase its first “Molecule of the Year”.

Why is Taq polymerase added last in PCR?

According to my observation, Taq Polymerase is added at the end because it used to be in small amount as mentioned earlier and it used to be sensitive to pH. So to give it optimum environment to preserve it for longer time in the solution….

Where is Taq polymerase found?

…a heat-stable DNA polymerase called Taq, an enzyme isolated from the thermophilic bacterium Thermus aquaticus, which inhabits hot springs.

How do you isolate Taq polymerase?

Taq DNA polymerase purification summary. The lysis mixture was incubated at 75°C for 60 min to Pluthero’s method, and 30 min to our method, and then centrifuged to get cleared lysate. The cleared lysate was treated with DNase I, and incubated at 75°C for another 30 min, and then centrifuged.

Why is Taq polymerase heat-stable?

Taq polymerase is naturally found in a thermophilic bacterium known as Thermus aquaticus. The bacterium lives in extremely hot environments such as hydrothermal vents and hot springs. Therefore, it is highly thermostable. The optimum temperature for the activity of Taq polymerase is 75-80 °C.

How do you make Taq polymerase?

Tips and suggestions for making home-made Taq polymerase

  1. You’ll need a plasmid then. Don’t have a Taq expression plasmid?
  2. Getting a high yield.
  3. Keeping it stable.
  4. (Very) easy removal of unwanted proteins.
  5. Be careful with the precipitation.
  6. Adjust the final concentration.
  7. Store it properly.
  8. Combat proteases.