Helpful guidelines

What does Alamar Blue detect?

What does Alamar Blue detect?

alamarBlue Cell Viability Reagent is an indigo-colored, non-toxic reagent that detects metabolically active cells and is used for the quantitative analysis of cell viability and proliferation.

How do you use Alamar Blue?

Just add the ready-to-use alamarBlue solution to the cells, incubate for at least 1-4 hours at 37°C between pH 6.8 and pH 7.4. Read the fluorescence or absorbance to measure cell respiration as an indicator for proliferation and cytotoxicity.

How long does Alamar Blue Last?

12 months
alamarBlue® should be stored in the dark, since the compound is light sensitive (Table 2). The product may be stored for 12 months at room temperature. This expiration date is given on the product label.

How do you make Blue Alamar?

All Answers (1)

  1. Dissolve alamar blue in PBS (pH 7.4) to 0.15 mg/ml.
  2. Filter-sterilize the resazurin solution through a 0.2 μm filter into a sterile, light protected container.
  3. Store the resazurin solution protected from light at 4°C for frequent use or at -20°C for long term storage.

How is Alamar Blue reduced?

The oxidation-reduction potential of Alamar Blue is +380 mV at pH 7.0, 25 °C. Alamar Blue, therefore, can be reduced by NADPH (Eo = 320 mV), FADH (Eo = 220 mV), FMNH (Eo = 210 mV), NADH (Eo = 320 mV), as well as the cytochromes (Eo = 290 mV to +80 mV).

What is microplate Alamar blue assay?

The 96-well Microplate Alamar Blue Assay (MABA) allows for the quantitative determination of drug susceptibility against any strain of replicating Mycobacterium tuberculosis to be completed within a week at minimal cost.

Does FBS interfere with luminescence?

In-house studies indicated samples with protein concentrations equivalent to 10% fetal bovine serum (FBS) did not interfere with the assay. However, Page et al. (1993) reported that serum may cause some quenching of fluorescence and recommended using the same serum concentration in controls to take this into account.

What are the 3 main groups of cells that proliferate?

(1) well-differentiated neurons and cells of skeletal and cardiac muscles that rarely divide and reproduce; (2) progenitor or parent cells that continue to divide and reproduce, like blood, skin, and liver cells; (3) undifferentiated stem cells that can enter the cell cycle and produce large number of progenitor cells …

How do you perform LDH assay?

To perform the assay, an aliquot of the cell culture medium is transferred to a new plate and the reaction mixture is added. After a 30-minute incubation, the reaction is stopped by adding Stop Solution and absorbance is measured using a microplate reader.