Is DTT hazardous?
Exposure can cause headache, weakness, restlessness, paralysis and coma. Exposure may cause irritation and possible burns to the skin. May cause dermatitis. Contact with the eye may cause sever irritation, and possible eye burns.
Why is DTT harmful?
The thiol-containing compound Dithiothreitol (DTT) has been shown to be toxic to cultured cells by inducing the generation of reactive oxygen species that ultimately cause cell death.
Is DTT flammable?
Not flammable or combustible. Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide.
What is DTT used for?
Dithiothreitol (DTT) is a redox reagent also known as Cleland’s reagent. It is used to break down protein disulfide bonds and stabilize enzymes and other proteins.
Why is DTT used in SDS-PAGE?
DTT is oftentimes used along with sodium dodecylsulfate in SDS-PAGE to further denature proteins by reducing their disulfide bonds to allow for better separation of proteins during electrophoresis. Because of the ability to reduce disulfide bonds, DTT can be used to denature CD38 on red blood cells.
Where is the primary site of insecticidal action of DTT?
DTT penetrates the insect body through the cuticle.
What is DL DTT?
General description An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.
Is Coomassie Blue toxic?
Coomassie Briliant Blue R-250 Dye (6104-59-2) Potential Adverse human health effects and symptoms : Non-toxic if swallowed (LD50 oral, rat > 5000 mg/kg). Not irritant to skin. Not irritant to eyes.
What is DTT solution?
Dithiothreitol (DTT) is a redox reagent also known as Cleland’s reagent. It is used to break down protein disulfide bonds and stabilize enzymes and other proteins. DTT is a small molecule and is an epimeric compound of dithioerythritol (DTE) These reducing reagent products are readily supplied by AG Scientific, Inc.